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KMID : 1094720110160010107
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 1 p.107 ~ p.113
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Enzymatic transformation of 2-O-α-D-glucopyranosyl-L-ascorbic acid by α-cyclodextrin glucanotransferase from recombinant Escherichia coli
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Zhang Zichen
Li Jianghua
Liu Long
Sun Jun
Hua Zhaozhe
Du Guocheng
Chen Jian
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Abstract
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The study aimed to produce 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) via the transglycosylation reaction by α-cyclodextrin glucanotransferase (α- CGTase) from recombinant Escherichia coli with L-ascorbic acid (AA) and β-cyclodextrin (β-CD) as the substrates. Liquid chromatography-tandem mass spectrometry analysis was conducted for AA-2G identification, and the glucoamylase treatment was carried out to produce AA-2G from AA-2-oilgosaccharides. The optimal temperature and pH for the enzymatic AA-2G production were 37°C and 5.5, respectively, and the optimal α-CGTase concentration and substrate mass ratio (AA:β-CD) for AA-2G synthesis were 160 U/mL and 1:1, respectively. At these optimal process conditions, maximal AA-2G production reached 13 g/L. This is the first report regarding the process optimization of enzymatic AA-2G production by α-CGTase from recombinant E. coli. The results may be useful for the industrial scale production of AA-2G.
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KEYWORD
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L-ascorbic acid, β-cyclodextrin, α-cyclodextrin glucanotransferase, 2-O-α-D-glucopyranosyl-L-ascorbic acid
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